Blood sugar levels
Venous blood is actually withdrawn using an effective Teflon catheter from the ear canal marginal vein, and you can blood sugar top was measured having fun with industrial strips and you may a glucose meter (Accu-Chek ® Productive, Roche, Germany).
Statistical study
All data is presented as mean ± SEM. Statistical analysis of data was performed with GraphPad Prism (version 6.07 for Windows, GraphPad Software, San Diego California, USA). Non-normally distributed variables: body weight, hematocrit, SD1, SD2, LF, HF, and LF/HF ratio. Normally distributed variables: blood glucose, heart rate, cardiac cycle duration, cardiac contraction time, systolic pressure, diastolic pressure, HRV total power. Mean arterial pressure as well as HRV power bands were normally distributed before but not after vagotomyparison between two groups was performed using parametric (Student?s t-tests) or nonparametric (Mann–Whitney or Wilcoxon test) tests, according to the data structureparison between more than two groups was performed using nonparametric analysis of variance (Kruskal–Wallis) test. Statistical decision level was set at p < 0.05.
Abilities
The mean body weight of control animals (1.87 ± 0.08 kg; n = 11) was like the initial mean weight of animals exposed to CNH (1.79 ± 0.10 kg; n http://www.datingranking.net/social-media-dating = 8). After 14 days, the body weight of control rabbits increased significantly to 2.27 ± 0.10 kg (p < 0.05). In contrast, the average body weight of CNH exposed rabbits was not significantly modified after 2-wks of CNH exposure (1.79 ± 0.10 kg vs. 1.78 ± 0.11 kg). Moreover, at the end of the CNH exposure rabbits had lower body weights than predicted values based on unexposed rabbits (2.27 ± 0.10 kg, p < 0.01).
CNH affected hematocrit but did not alter blood glucose levels. The mean hematocrit increased from 40.3 ± 0.7% at the beginning of the CNH period to 55.8 ± 0.9% after 14 days of CNH (p < 0.001). The mean blood glucose levels, measured after anesthetic induction and prior to physiological recordings, were similar in control and CNH rabbits (7.99 ± 0.51 mmol/L, n = 5 and 8.55 ± 0.47 mmol/L, n = 5, respectively; p > 0.05).
Negative effects of CNH to the sleep cardiovascular details from inside the basal standards
Table step 1 has the imply viewpoints for the cardio details to own control and you will CNH rabbits counted while in the good 10-min recording away from straight heart sounds after the induction of anesthesia. Rabbits confronted with 2 weeks out-of CNH had no mathematically tall variations (p > 0.05) in the arterial pressure, heart rate, cardiac duration cycle and you will contraction big date, as compared to control rabbits. Particularly, pulse rate and mean arterial in addition to systolic and you will diastolic arterial challenges was in fact a bit but not notably increased from inside the CNH rabbits compared to the opinions in control rabbits (Dining table 1). Furthermore, arterial pulse tension are some although not somewhat lower in CNH rabbits with regards to handle pets (Table step 1).
Cardio parameters immediately following two-sided vagotomy
Most cardiovascular variables were affected by bilateral vagotomy (Table 2) in control rabbits. After vagotomy, mean heart rate increased significantly from ± bpm to ± bpm (p < 0.05; Fig. 2a). Similarly, after vagotomy, mean systolic, diastolic, and pulse arterial pressure increased (p < 0.05), and mean arterial blood pressure also increased (p < 0.05). Conversely, bilateral vagotomy did not modify significantly these cardiovascular variables in the CNH rabbits (Table 2).
Heart rate before and after bilateral vagotomy in control and CNH rabbits. a Mean heart rate was significantly increased in control (n = 6) but not in CNH rabbits (n = 6) after bilateral vagotomy (VgTexas). b Scatter plot of mean heart rate after bilateral vagotomy as a function of basal mean heart rate. Dotted line: line of identity. c In control rabbits, changes in mean heart rate were maximal in the midrange of basal heart rate; in CNH rabbits, changes were inversely and linearly related to basal mean heart rate. Control rabbits: empty circles, continuous line. CNH rabbits: filled circles, segmented line. Bars: SEM. *p < 0.05
